Fig. 6
From: Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model
SAC and AGE attenuates CoCl2-induced overproduction of ROS and HIF-1α stabilization and binding activity. a Cells were incubated with CoCl2 and SAC or AGE to determine intracellular ROS levels. Data are shown as the mean ± S.E.M. n = 6. *p < 0.001 vs. vehicle. #p < 0.05, ##p < 0.01 and ###p < 0.001 vs. the same concentration of CoCl2. b Binding of stabilized HIF-1α to HRE sequences was determined using an ELISA. Data are shown as the mean ± S.E.M. n = 3. Two-way ANOVA followed by Bonferroni comparisons. *p < 0.001 vs. vehicle; #p < 0.001 vs. the same concentration of CoCl2