Fig. 5
From: Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model
Protective effect of SAC and AGE on CoCl2-induced apoptosis. Upper panel: Representative figures of Annexin and 7-AAD double staining assay using flow cytometry after 48-h incubation with CoCl2 and SAC or AGE (a–f). Percentage of 7-AAD + (g), live Annexin-/7AAD- (h), early apoptotic Annexin +/7AAD- (i) and late apoptotic plus necrotic cells Annexin +/7AAD + (j). Data are shown as the mean ± S.E.M. n = 6. Two-way ANOVA followed by Bonferroni comparisons. *p < 0.05, **p < 0.001 vs. control cells (vehicle), #p < 0.05, ##p < 0.01 vs. the same concentration of CoCl2