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Fig. 2 | Biological Research

Fig. 2

From: Aged garlic extract and S-allylcysteine prevent apoptotic cell death in a chemical hypoxia model

Fig. 2

Effect of CoCl2 on HIF-1α stabilization and binding activity. Cells were incubated for 24 or 48 h with 0.5 or 1.0 mM CoCl2. Binding of nuclear HIF-1α to HRE sequences was determined using an ELISA. Data are shown as the mean ± S.E.M, n = 3. Two-way ANOVA followed by Bonferroni comparisons. *p < 0.05 and **p < 0.001 vs. vehicle at the same time point, #p < 0.001 vs. 0.5 mM CoCl2 at the same time point

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