Fig. 3

Effect of RPRM overexpression on migration and invasion properties in MDA-MB-231 cells. a qPCR and Western Blot of MDA-MB-231 cells with overexpression of RPRM (MDA-MB-231-RPRM) and empty control (MDA-MB-231-pCMV6). The antibody used was a monoclonal antibody anti-DDK. Reprimo protein (12kD) is detected as 23–25 kD approximately due to glycosylation. b Overexpression of RPRM in MDA-MB-231 cells negatively regulates the migration ability. Representative micrographs of transwell migration assay were selected to show each condition (40× magnification). Quantification of cell migration expressed as the percentage of control. c Overexpression of RPRM in MDA-MB-231 reduced wound healing ability. Representative micrographs were taken at 0, 12, 24, 36 and 48 h (10× magnification). d Overexpression of RPRM in MDA-MB-231 cells significantly reduced the invasion at 24 h using a matrigel-coated insert. Representative images of invaded cells were selected to show each condition (40× magnification). Quantification of cell invasion expressed as the percentage of control. For each assay values were compared to control (MDA-MB-231-pCMV6) using a Mann–Whitney test. The error bars indicate standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001