Fig. 2
From: Reprimo as a modulator of cell migration and invasion in the MDA-MB-231 breast cancer cell line
mRNA expression and promoter methylation of RPRM in breast cancer cell lines. a Aberrant methylation was found in 3/5 of breast cancer cell lines studied. M methylated status, U unmethylated status. PCR product = 112 pb. b Expression levels before and after treatment with 5′-Aza-dC, by RT-PCR. Cells were treated with 5′-Aza-dC (5 μM) for 5 days. In two (MDA-MB-231 and BT-20; lane 1 and 2, respectively) out of three BC cell lines the expression was decreased by methylation, and restored after 5′Aza-dC treatment. RPRM PCR product = 137; 18S PCR product = 102 pb