TY - JOUR AU - Beltran, Sebastian AU - Munoz-Bergmann, Cristian A. AU - Elola-Lopez, Ana AU - Quintana, Javiera AU - Segovia, Cristopher AU - Trombert, Annette N. PY - 2016 DA - 2016/01/07 TI - The expression of heterologous MAM-7 in Lactobacillus rhamnosus reduces its intrinsic capacity to inhibit colonization of pathogen Vibrio parahaemolyticus in vitro JO - Biological Research SP - 2 VL - 49 IS - 1 AB - Vibrio parahaemolyticus (V. parahaemolyticus) is a Gram-negative, halophilic bacterium recognized as one of the most important foodborne pathogen. When ingested, V. parahaemolyticus causes a self-limiting illness (Vibriosis), characterized mainly by watery diarrhoea. Treatment is usually oral rehydration and/or antibiotics in complicated cases. Since 1996, the pathogenic and pandemic V. parahaemolyticus O3:K6 serotype has spread worldwide, increasing the reported number of vibriosis cases. Thus, the design of new strategies for pathogen control and illness prevention is necessary. Lactobacillus sp. grouped Gram positive innocuous bacteria, part of normal intestinal microbiota and usually used as oral vaccines for several diarrheic diseases. Recombinants strains of Lactobacillus (RL) expressing pathogen antigens can be used as part of an anti-adhesion strategy where RL block the pathogen union sites in host cells. Thus, we aimed to express MAM-7 V. parahaemolyticus adhesion protein in Lactobacillus sp. to generate an RL that prevents pathogen colonization. SN - 0717-6287 UR - https://doi.org/10.1186/s40659-015-0064-1 DO - 10.1186/s40659-015-0064-1 ID - Beltran2016 ER -