Table 2 Primer pairs used for gene amplification in bovine mammary epithelial cells
Gene | Accession number | Primer pairs (5′- end to 3′- end) | Product length (bp) |
|---|---|---|---|
α-SMA | NM_001034502 | For: GGTGATGAAGCACAAAGCAA Rev: TGAGAAGGGTTGGATGCTCT | 154 |
Vimentin | NM_173969.3 | For: CGCTCAAAGGGACTAACGAG Rev: TGACATTCAGCAGGTCTTGG | 174 |
CK7 | NM_001046411.1 | For: TTACCAGACCAAGTTTGA Rev: ATCTCATTCCGGGTATTC | 78 |
CK18 | NM_001192095.1 | For: ATTGATAATGCCCGTCTTGC Rev: AGCCTCGATCTCAGTCTCCA | 156 |
CK19 | NM_001015600.3 | For: GATGACTTCCGCACCAAGTT Rev: AGCAGAATCCACCTCCACAC | 234 |
Beta actin | XM_006715764.1 | For: AACTCCATCATGAAGTGTGACG Rev: GATCCACATCTGCTGGAAGG | 234 |
Ubiquitin | see [39] | For: TTCACAGGTCAAAATGCAGA Rev: ATCTGCATACCACCCCTCAG | 237a |