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Fig. 2 | Biological Research

Fig. 2

From: Can widely used cell type markers predict the suitability of immortalized or primary mammary epithelial cell models?

Fig. 2

Flow cytometry analysis of selected markers of mesenchymal-like cells in primary and immortalized mammary cultures. a. Cell distribution pattern in MAC-T, bMECUS and bMECCH cultures based on the forward scatters. b. The cell staining for vimentin and α-smooth muscle actin (α-SMA) in MAC-T, bMECUS and bMECCH cultures, respectively, is shown in red. Two sets of control stainings were included: (i) without primary and secondary Ab (small dashed lines) and (ii) with IgG1 isotype and secondary Ab (large dashed lines). The staining was acquired by counting at least 20,000 events

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