Fig. 1

The expression of MCP-1-CCR2 axis genes and cartilage matrix markers after stimulation of MCP-1. a, b In OA chondrocytes, after stimulation of MCP-1 in culturing medium of the relative expression of MCP-1 and CCR2 mRNA increased significantly compared with unaffected OA chondrocytes; c, d the expression of Mcp-1 in OA/CHON and OA/S.F were significantly higher than RA/SF and WT/CHON. The expression of Ccr2b in OA/CHON were significantly lower than that in OA/S.F, RA/SF and WT/CHON. MCP-1 Stim, MCP-1 stimulated; RA/SF, RA synovial fibroblasts; OA/S.F, OA synovial fibroblast; OA/CHON, OA chondrocytes; WT/CHON, wild-type (normal controls) chondrocytes; RA/CHON, RA chondrocytes; and e, f, g after stimulation of MCP-1, the expression of MCP-1 and MMP13 protein increased significantly than unstimulated normal OA chondrocytes. MMP3 increased but there was not significantly higher than unstimulated normal chondrocytes. MCP-1 Stim, MCP-1 stimulated OA chondrocytes; N–N, unstimulated wild-type (normal controls) chondrocytes; N–M, MCP-1 stimulated wild-type (normal controls) chondrocytes. *P < 0.05, **P < 0.01, ***P < 0.001