Table 1 Primer sequences of real-time PCR
From: Tridax procumbens flavonoids promote osteoblast differentiation and bone formation
Gene | Forward | Reverse |
|---|---|---|
Alkaline phosphatase | 5′ ACAGCCATCCTGTATGGCAA | 3′ GCCTGGTAGTTGTTGTGAGCA |
Osteocalcin | 5′ TGAGGACCATCTTCTGCTCA | 3′ TGGACATGAAGGCTTTGTCA |
Osterix | 5′ TATGCTCCGACCTCCTCAACT | 3′ TCCTATTTGCCGTTTTCCCGA |
Runx2 | 5′ GATCTGAGATTTGTAGGCCG | 3′ TCATCAAGCTTCTGTCTGTGCC |
BMP-2 | 5′ CGGACTGCGGTCTCCTAA | 3′ GGGAAGCAGCAACACTAGA |
BMP-4 | 5′ GACTTCGAGGCGACACTTCT | 3′ GCCGGTAAAGATCCCTCATGTA |
BMP-7 | 5′ GAAAACAGCAGCAGTGACCA | 3′ GGTGGCGTTCATGTAGGAGT |