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Table 5 Primers used for validation analysis

From: Transcriptome responses to heat- and cold-stress in ladybirds (Cryptolaemus montrouzieri Mulasnt) analyzed by deep-sequencing

Gene name Gene ID Primer
Heat-responsive protein 12 Locus_1903 5′-TTATCCAAACCGAGAACACCG-3′
5′-GAATGTGCTCCGAAACCTGTG-3′
Casein kinase beta polypeptide Locus_1270 5′-TGGGTTTATCTGATGTTCCTGG-3′
5′-CGGTATGATGGTGACGTGATG-3′
Stress-induced-phosphoprotein 1 Locus_1379 5′-GTATGGAAACTGCCGGTATTGG-3′
5′-GCGGGATCTCTAAGTATTTGTTGC-3′
Ribose-phosphate pyrophosphokinase Locus_1071 5′-AGGCTTAACGTCGAGTTTGCC-3′
5′-GTCCTTGACATCTCCCACTAATACC-3′
Cytochrome p450 Locus_2703 5′-CATGGTTCACAGCGTGTAATAGC-3′
5′-GTGCCTTAGGCAAACGTCAAAT-3′
Chitinase 3 Locus_53 5′-GACACTATGCACCTCTGAACGC-3′
5′-CCAGTAAGTGATACCTCGGAAAAC-3′
Novel protein zgc 112389 Locus_15691 5′-TTTAGGTTCTCCACTATGGCTAC-3′
5′-AGTAATCACAGCAACAGCCAAT-3′
Serine protease P80 Locus_10683 5′-TTTGAAACTCGAAAGGGCATTG-3′
5′-GAATAGGATAGACGAGCAAGG–3′
NTF2 Locus_15496 5′-AAAGGAGACACTTAATTTCCAGG-3′
5′-TCCCATTACACCATTACCATTC-3′
Macrophage-stimulating protein receptor Locus_23091 5′-TTTCTCGTGGGACAAGATTACC-3′
5′-GAGTCTCGCAGTTAGGTCTTTCAC-3′
Acyl carrier protein Locus_2931 5′-ATTCGCTGGATCATGTTGAAGT-3′
5′-GCTTCTCAGCATCGGCATCT-3′
ATPase WRNIP1 Locus_927 5′-CTGAGGCTTCTCCTGCTAAACG-3′
5′-CCATTCAAGGCAGGCGATTT-3′
Beta-tubulin Locus_627 5′-CACGGAAGGTACTTGACTGTTG-3′
5′-GCTGCTGTTCTTGTTTTGGATG-3′