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Table 1 Major characteristics of DGE libraries and tag mapping to the reference transcripts database

From: Transcriptome responses to heat- and cold-stress in ladybirds (Cryptolaemus montrouzieri Mulasnt) analyzed by deep-sequencing

 

NC

HS

CS

Distinct tag

Total tag

Distinct tag

Total tag

Distinct tag

Total tag

Raw data

120164

6134223

133704

5798954

117658

5901493

Tags containing N

678

1963

713

2119

606

2092

Adaptors

43

77

41

71

36

65

Tag CopyNum <2

68023

68023

75048

75048

65218

65218

Clean tag

51420

6064160

57902

5721716

51798

5834118

CopyNum >1

51420

6064160

57902

5721716

51798

5834118

CopyNum >5

23386

5984211

25732

5629564

23338

5753027

CopyNum >10

16051

5928384

17181

5564674

15665

5694782

CopyNum >20

10615

5848664

10970

5473325

10123

5613306

CopyNum >50

5526

5684688

5374

5295392

4920

5446060

CopyNum >100

3022

5507459

2885

5120118

2531

5277864

Tag mapping

      

All mapping

23180

1365846

27457

1235061

23818

1030557

Unambiguous mapping

22967

1343506

27245

1224209

23620

1019908

Unknown tag

28240

4698314

30445

4486655

27980

4803561

  1. All Mapping represents the number of all tags mapped to the reference transcripts database, Unambiguous Mapping represents the number of unambiguous tags mapped to the reference transcripts database, unambiguous tags indicate the tags matched only to one gene