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Table 1 Major characteristics of DGE libraries and tag mapping to the reference transcripts database

From: Transcriptome responses to heat- and cold-stress in ladybirds (Cryptolaemus montrouzieri Mulasnt) analyzed by deep-sequencing

  NC HS CS
Distinct tag Total tag Distinct tag Total tag Distinct tag Total tag
Raw data 120164 6134223 133704 5798954 117658 5901493
Tags containing N 678 1963 713 2119 606 2092
Adaptors 43 77 41 71 36 65
Tag CopyNum <2 68023 68023 75048 75048 65218 65218
Clean tag 51420 6064160 57902 5721716 51798 5834118
CopyNum >1 51420 6064160 57902 5721716 51798 5834118
CopyNum >5 23386 5984211 25732 5629564 23338 5753027
CopyNum >10 16051 5928384 17181 5564674 15665 5694782
CopyNum >20 10615 5848664 10970 5473325 10123 5613306
CopyNum >50 5526 5684688 5374 5295392 4920 5446060
CopyNum >100 3022 5507459 2885 5120118 2531 5277864
Tag mapping       
All mapping 23180 1365846 27457 1235061 23818 1030557
Unambiguous mapping 22967 1343506 27245 1224209 23620 1019908
Unknown tag 28240 4698314 30445 4486655 27980 4803561
  1. All Mapping represents the number of all tags mapped to the reference transcripts database, Unambiguous Mapping represents the number of unambiguous tags mapped to the reference transcripts database, unambiguous tags indicate the tags matched only to one gene