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Fig. 3 | Biological Research

Fig. 3

From: Functional disruption of human leukocyte antigen II in human embryonic stem cell

Fig. 3

CIITA and HLA Class II Expression in fibroblasts derived from CIITA targeted hESCs. a RT-PCR analysis of β2M, CIITA, HLA II (DRA, DQA, DPA) and Ii in hESCs-derived fibroblasts. They were treated with IFN-γ (500 U/ml) for 5 days. The control groups were IFN-γ free. All groups were compared with CIITA +/+ IFN-γfree group. Significance was assessed by a t test. The data are expressed as the mean ± SEM. n ≥ 3. *** p < 0.001, ** P < 0.01. Western blotting (b) and immunostaining (c) analysis of HLA II and CIITA proteins expression in fibroblasts (fibroblasts treated as mentioned above). Scale bar 100 μm. d FACS analysis of HLA I and II proteins expression on cell surface in fibroblasts (fibroblasts treated as mentioned above)

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