Fig. 2
From: Functional disruption of human leukocyte antigen II in human embryonic stem cell
The pluripotency of CIITA targeted hESCs. a Immunostaining of pluripotent markers, Nanog, Oct4, SSEA3 and Tra-1-60 in CIITA −/− hESCs. b HE staining identified three germ layers [mesoderm (left), ectoderm (middle) and endoderm (right)] in teratomas formed from CIITA −/− hESCs. Scale bar 100 μm. c RT-PCR analysis of differentiated markers expression in CIITA-targeted hESC-derived EBs. d Karyotype analysis of CIITA heterozygous and homozygous hESCs. Both groups had two samples been analyzed and no abnormal karyotype was found