Fig. 2

Effects of glutamate, (+)-pentazocine, and haloperidol on the NADPH oxidase activity in membrane preparations of KO and Wt mice brains. Membrane preparations were incubated with glutamate (Glu), haloperidol (Halo), with (+)-pentazocine (Pent) or with glutamate and both sigma-ligands (Glu + Halo + Pent) and NADPH oxidase activity determined as described in “Methods”. Data represented are mean ± SEM of results from four separate experiments performed in duplicate. *P < 0.05, t test compared with corresponding control (c)