Fig. 1

Western blot analysis of SigR1, BiP, Rac1, IP3R and NR2B in membrane preparations of KO and Wt mice brains. Total SigR1 and BiP were analyzed directly in membrane preparations. For the co-immunoprecipitation experiments solubilized membrane preparation were immunoprecipitated with anti-Sig1R, the immunoprecipitate was subjected to SDS-PAGE and transferred. a The blots were probed to Western analysis with antibodies against Rac1 (Wb: Rac1), BiP (Wb: BiP), IP3R (Wb: IP3R) and NR2B (Wb: NR2B). β-Actin was also visualized by Western blotting to confirm equal loading of the fractions. The blot is representative of four similar experiments. b Quantification of blots shown in B; n = 4. *P < 0.05, compared with control (Wt) levels. **P > 0.05, compared with control (Wt) levels