Skip to main content
Fig. 4 | Biological Research

Fig. 4

From: Development of a novel method to evaluate sialylation of glycoproteins and analysis of gp96 sialylation in Hela, SW1990 and A549 cell lines

Fig. 4

Expression, biotinylation, and sialylation of gp96 in different cancer cell lines. a The expression of gp96 mRNA in different cancer cell lines was determined using real-time PCR and normalized to GADPH expression. b The expression of gp96 protein and amount of biotinylated gp96 obtained after purification from different cancer cell lines were determined using western blot analysis. β-actin served as an internal control. c The variation in the degree of biotinylation of gp96 was determined using ELISA. d The variation in the degree of sialylation of gp96 was determined using ELISA

Back to article page
\