Skip to main content
Fig. 3 | Biological Research

Fig. 3

From: Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia

Fig. 3

Angiogenesis assay measuring tube formation ability of HUVECs with conditioned medium from ARPE-19 cells. a Representative photomicrographs. Typical tube formation was not seen in any of the samples with conditioned medium collected from cells cultured at temperatures from 37 to 31°C after 24 and 48 h. Significant differences were observed in the number of branching points and total skeleton length (arrows) among each of the conditions. Bar 50 μm. b Quantitative analysis of the number of branching points and total skeleton length. Data are presented as mean ± SD (n = 4). *P < 0.05, **P < 0.01.

Back to article page

Biological Research

ISSN: 0717-6287

Contact us