Sortin2 enhances internalization of FM4-64. a Diagram of FM4-64 endocytosis labeling. Cells were incubated with 24 μM FM4-64 for 30 min at 4°C for plasma membrane FM4-64 binding. Then turned to 28°C (time 0) to be imaged subsequently at different times of incubation by confocal microscopy. Progressively FM4-64 is progressively trafficking to intracellular compartments by endocytosis. b
S. cerevisiae parental line was grown on YPD 1% DMSO (control) and YPD supplemented with 20 μM Sortin2. Two representative images of approximately 25 cells are shown in each condition. The experiment was performed three times. Scale bar represents 5 μm. c Cells with FM4-64 labeled vacuoles were scored in each condition on b. The percentage of cells with FM4-64 labeled vacuoles is informed with standard deviation.