Formation of neurospheres in response to AF. a The comparative sizes of neurospheres were measured by flow cytometry. FSC-H indicates the height of forward scatter (FSC), which is proportional to cell size. The R1 area represents enlarged neurosphere (CTL, 1.33; 10 μL/mL AF, 2.79; 15 μL/mL AF, 3.61). b Representative pictures at 5 days after treatment with vehicle or AF. c Statistical analysis on the formation of neurosphere. The AF-treated cells showed a significant increase in the diameter of neurosphere comparing to control cells. ***p < 0.001 versus CTL cells (CTL). Scale bar indicates 100 μm.