Promotion of neural cell lineage in response to AF. a The nuclei of cells were stained with Hoechst 33342 (blue). The clustered cells were stained with immature neuronal marker, Tuj-1 (green). Scale bar indicates 60 μm. b Western blot from the cortical neurons cultured for 5 days after treatment with vehicle or AF. AF-treated cells showed a significant increase in immature neuronal marker, Tuj-1. *p < 0.05, versus CTL cells (CTL).