Figure 3
Effect of gabapentin on evoked excitation in the Sp5c. a Recording made during superfusion with control mock CSF. The left panels represent neural activity, which is indicated as changes in fluorescence intensity using pseudocolor. The right panel shows the time course of the signal change and time of electrical stimulation. Optical image in the left panel shows 165 ms after stimulation, as indicated by the vertical dotted line in the right panel. Two horizontal lines in the image are the anchors for slice preparation. b Recording made during superfusion with mock CSF containing 100 μM gabapentin. c Peak amplitudes of evoked excitation in the Sp5c (indicated by arrows in the right panels of a, b) were measured during superfusion with 1, 10 and 100 μM gabapentin. d Fluorescence signal amplitudes at 165 ms after stimulation (indicated by arrows in the right panels of a, b) were measured during superfusion with 1, 10 and 100 μM gabapentin. e Fluorescence signal amplitudes at 385 ms after stimulation (indicated by arrows in the right panels of a, b) were measured during superfusion with 1, 10 and 100 μM gabapentin. In each graph, fluorescence signal amplitude is indicated as the percent amplitude of the control value during superfusion with control mock CSF. Data of each concentration were obtained from six preparations and are presented as mean ± SD. Administration of 1 and 10 μM gabapentin did not induce significant changes in evoked excitation in the Sp5c, but 100 μM gabapentin emphasized the evoked excitation. **P < 0.01; NS not significant.