Effect of DC on morphological studies, DNA fragmentation and cell cycle arrest in FasL- induced apoptotic cell death in HeLa cells. HeLa cells were treated with indicated concentrations of DC (0.5 µg/mL) and FasL (50 ng/mL) as described in the methods. a The cells were fixed and stained with DAPI solution. The stained nuclei were the observed under a fluorescent microscope (×400). b The percentage of apoptotic cells was quantified. c DNA fragmentation assay. Same amount of genomic DNA (3 µg) extracted from cells was separated by 2.0% agarose gel electrophoresis, and visualized under UV light after staining with EtBr. Marker indicates a size marker of the DNA ladder. d To evaluate the degree of apoptosis reduced by DC, cells were evaluated by Flow Cytometry for sub-G1 DNA content (hypodilpoid DNA), which represents the cells undergoing apoptotic DNA degradation. Data are the mean ± SEM (n = 3). The significance was determined by Student’s t-test. #
p < 0.05 compared with control group and **p < 0.01, compared with FasL treated group. DC doxycycline.