Fig. 2

Effect of DC on hydrogen peroxide (H₂O₂)—or cisplatin-induced apoptotic cell death in HeLa cells. a HeLa cells were pretreated with indicated concentrations of DC (0.01–16 µg/mL) for 12 h with or without H₂O₂ (1.5 mM) for 24 h. b HeLa cells were pretreated with indicated concentrations of DC (0.01–16 µg/mL) for 12 h with or without cisplatin (40 µM) for 24 h. The cell viability was measured by the MTT assay. Each point represents the mean ± SEM (n = 3). The significance was determined by Student’s t-test. ^# p < 0.05 when compared with control group. ^$No significant difference when compared with H₂O₂ or cisplatin treated groups. DC doxycycline.