Fig. 7

Human recombinant leptin activate endothelial cells. Images of CAM stained for CD34 under 3 μg leptin for 72 h. Control CAM treated with 1X PBS for 72 h. Control (a) shows the expression of CD34 protein at the lumen of the blood vessel and CAM treated with 3 μg leptin shows the expression at the capillary plexus or blood sinus area as coiled structure where endothelial cells pooled out (b, d) and also at those endothelial cell which is present at the lumen of the blood vessel (c, e). Images were recorded using light microscope. Arrow indicates the presence of CD34 protein, magnification 40X and bar is 50 μM