Fig. 6

Human recombinant leptin enhances the gelatinase activity of MMP2. a Image represents the gelatin zymography of MMP2 activity from CAM after treated with 1, 3 and 5 μg of HRL and control with 1X PBS for 72 h. The image shows that the gelatinase activity was increased under 3 and 5 μg of HRL which is visible as white band against black background. Image is the result of 3 set of experiments. b MMP2 gelatinase activity was measured as OD value using Image J software. Percentage of MMP2 gelatinase activity increased significantly under 3 and 5 μg of HRL with higher percentage under 3 μg of HRL. Each value is the mean ± SEM, *p = <0.001 versus control, n = 3. (Lane 1- protein weight marker, Lane 2 - control, lane 3–1 μg HRL, lane 4–3 μg HRL and lane 5–5 μg HRL)