Fig. 5

Human recombinant leptin enhances the mRNA level expression of VEGF165, VEGF121, bFGF2, MMP2 and MMP9. Images of Reverse Transcriptase- PCR products of (a) VEGF165 (b) VEGF121 (c) bFGF2 (d) MMP2 and (e) MMP9 from CAM after treated with 1, 3 and 5 μg of HRL and control with 1X PBS for 72 h. GAPDH was used as internal loading control. The transcripts were confirmed by 100bp DNA ladder. Graph represents the OD value ratio of mRNA transcripts after normalizing with GADPH OD value of the same. The OD value was measured using Image J software. The relative level expression of specified genes were increased significantly for 3 μg of HRL and under 5 μg of HRL the relative expression of VEGF 165 and MMP2 increased significantly after 72 h of incubation.. Each value is the mean ± SEM, *p= <0.001 versus control, n = 3. Experiments were performed in triplicate and data presented as mean + SEM. (Lane 1 - control, lane 2 – 1 μg HRL, lane 3 – 3 μg HRL and lane 4- 5 μg HRL)