Fig. 4

Human recombinant leptin increases the size of tissue by means of inducing new vessel growth. Total thickness of the tissue at the treated area with 1, 3 and 5 μg of HRL was measured from haematoxylin and eosin stained vertical cross section after 72 h. Control CAM was treated with 1X PBS. The distance between chorionic and allantoic epithelial layers was calculated morphometrically in μM. CAM treated with 3 μg of HRL shows significantly increased tissue thickness than other two concentrations. Material shrinkage is estimated to be ~25 % relative to the fresh material in all cases. Experiments were performed in triplicate and data presented as mean + SEM, (*p = <0.001versus control)