Figure 4From: Integrin-β1, not integrin-β5, mediates osteoblastic differentiation and ECM formation promoted by mechanical tensile strainAssay of Hydroxyproline (Hyp), GAG and Calcium (Ca) in ECMs. The mechanical strain increased Hyp content, and relative levels of GAG and Ca (the first and second column of A, B and C). Integrin-β1 siRNA reduced the content of Hyp, levels of GAG and Ca, which increased by the tensile strain. The effect of integrin-β5 siRNA on levels of hyp, GAG and Ca was nearly naught, the inhibitory effect of both integrin-β1 siRNA and integrin-β5 siRNA simultaneously on hyp, GAG, Ca was weaker than only integrin-β1 siRNA. *P < 0.05, **P < 0.01, between indicated groups.Back to article page