Sox2 negatively regulate HAMP2 expression in primary hepatocytes. Primary hepatocytes were cultured and lentivirus expressing Myc-Sox2 (A) or shRNA targeting Sox2 (B and C) infected the cells. Cells were harvested at 72 h later for Western blotting assay and RT-qPCR assay to determine Myc-Sox2 expression, HAMP expression and Sox2 knockdown efficiency. The experiments were repeated for three times with similar results and data were shown as means ± SE. Statistical analyses were conducted using a one-way ANOVA with Tukey’s multiple comparison test to determine individual p-values (B-C) or an unpaired, two-tailed student t-test (A). Significant differences are indicated by *p < 0.05.