Knockdown of Sox2 can further increase HAMP expression in Huh7 cells. (A) Huh7 cells were transfected with Sox2-targeting siRNAs and control siRNA. Western blot analysis of partial Sox2 knockdown cells was performed to evaluate the Sox2 knockdown efficiency. (B-C) Huh7 cells transfected with Sox2 siRNAs were treated with IL-6 or BMP-2 were subjected for RNA isolation to determine HAMP expression with RT-qPCR assay. The experiments were repeated for three times with similar results and data were shown as means ± SE. Statistical analyses were conducted using one-way ANOVA with Tukey’s multiple comparison test. Significant differences are indicated by *p < 0.05.