Table 1 Summary of P. aeruginosa PAO1 polyP synthesis mutants ( ppk1 and ppk2 ) common gain and lost phenotypes
Test | ppk1 difference | ppk2 difference | Mode of action |
|---|---|---|---|
Gain phenotype | Gain phenotype | ||
L-Arginine | 50 | 75 | C-Source, carboxylic acid |
His-Met | 61 | 56 | N-Source, peptide |
Carbenicillin | 158 | 71 | Wall, lactam |
Cefsulodin | 80 | 80 | Wall, cephalosporin |
Ruthenium red | 78 | 110 | Respiration, mitochondrial Ca2+ porter |
Erythromycin | 67 | 80 | Protein synthesis, 50S ribosomal subunit, macrolide |
Lost phenotype | Lost phenotype | ||
D-Gluconic acid | −112 | −106 | C-Source, carboxylic acid |
D-Mannitol | −68 | −57 | C-Source, carbohydrate |
L-Valine | −60 | −58 | N-Source, amino acid |
L-Methionine Sulfone | −69 | −95 | S-Source, organic |
Orphenadrine | −78 | −146 | Anti-cholinergic |
Patulin | −534 | −495 | Microtubulin polymerization inhibitor, antifungal |
2,2′-Dipyridyl | −401 | −397 | Chelator, lipophilic |
Sodium Arsenite | −332 | −242 | Toxic anion |
Sodium Arsenate | −305 | −321 | Toxic anion, P04 analog |
Dichlofluanid | −271 | −119 | Fungicide, phenylsulphamide |
Thiamphenicol | −269 | −193 | Protein synthesis, amphenicol |
Chloramphenicol | −209 | −173 | Protein synthesis, amphenicol |
Chlorodinitrobenzene | −182 | −187 | Oxidizes sulfhydryls, depletes glutathione |
8-Hydroxyquinoline | −181 | −230 | Chelator, lipophilic |
Nafcillin | −163 | −172 | Wall, lactam |
Antimony (III) chloride | −158 | −153 | Toxic cation |
1,10-Phenanthroline monohydrate | −152 | −171 | Chelator, lipophilic |
Captan | −152 | −113 | Fungicide, carbamate |
Sulfathiazole | −148 | −172 | Folate antagonist, PABA analog |
Gallic acid | −141 | −115 | Respiration, ionophore, H+ |
Poly-L-lysine | −140 | −63 | Membrane, detergent, cationic |
Protamine sulfate | −129 | −109 | Membrane, nonspecific binding |
Sulfadiazine | −127 | −107 | Folate antagonist, PABA analog |
Oxacillin | −124 | −130 | Wall, lactam |
Myricetin | −113 | −122 | DNA & RNA synthesis, polymerase inhibitor |
Nickel chloride | −113 | −80 | Toxic cation |
Plumbagin | −106 | −118 | Oxidizing agent |
Oxophenylarsine | −99 | −161 | Tyrosine phosphatase inhibitor |
trans-Cinnamic acid | −98 | −90 | Respiration, ionophore, H+ |
EDTA | −67 | −88 | Chelator, hydrophilic |
Glycine hydroxamate | −66 | −136 | tRNA synthetase |