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Table 1 Summary of P. aeruginosa PAO1 polyP synthesis mutants ( ppk1 and ppk2 ) common gain and lost phenotypes

From: Multiple antibiotic susceptibility of polyphosphate kinase mutants (ppk1 and ppk2) from Pseudomonas aeruginosa PAO1 as revealed by global phenotypic analysis

Test ppk1 difference ppk2 difference Mode of action
  Gain phenotype Gain phenotype  
L-Arginine 50 75 C-Source, carboxylic acid
His-Met 61 56 N-Source, peptide
Carbenicillin 158 71 Wall, lactam
Cefsulodin 80 80 Wall, cephalosporin
Ruthenium red 78 110 Respiration, mitochondrial Ca2+ porter
Erythromycin 67 80 Protein synthesis, 50S ribosomal subunit, macrolide
  Lost phenotype Lost phenotype  
D-Gluconic acid −112 −106 C-Source, carboxylic acid
D-Mannitol −68 −57 C-Source, carbohydrate
L-Valine −60 −58 N-Source, amino acid
L-Methionine Sulfone −69 −95 S-Source, organic
Orphenadrine −78 −146 Anti-cholinergic
Patulin −534 −495 Microtubulin polymerization inhibitor, antifungal
2,2′-Dipyridyl −401 −397 Chelator, lipophilic
Sodium Arsenite −332 −242 Toxic anion
Sodium Arsenate −305 −321 Toxic anion, P04 analog
Dichlofluanid −271 −119 Fungicide, phenylsulphamide
Thiamphenicol −269 −193 Protein synthesis, amphenicol
Chloramphenicol −209 −173 Protein synthesis, amphenicol
Chlorodinitrobenzene −182 −187 Oxidizes sulfhydryls, depletes glutathione
8-Hydroxyquinoline −181 −230 Chelator, lipophilic
Nafcillin −163 −172 Wall, lactam
Antimony (III) chloride −158 −153 Toxic cation
1,10-Phenanthroline monohydrate −152 −171 Chelator, lipophilic
Captan −152 −113 Fungicide, carbamate
Sulfathiazole −148 −172 Folate antagonist, PABA analog
Gallic acid −141 −115 Respiration, ionophore, H+
Poly-L-lysine −140 −63 Membrane, detergent, cationic
Protamine sulfate −129 −109 Membrane, nonspecific binding
Sulfadiazine −127 −107 Folate antagonist, PABA analog
Oxacillin −124 −130 Wall, lactam
Myricetin −113 −122 DNA & RNA synthesis, polymerase inhibitor
Nickel chloride −113 −80 Toxic cation
Plumbagin −106 −118 Oxidizing agent
Oxophenylarsine −99 −161 Tyrosine phosphatase inhibitor
trans-Cinnamic acid −98 −90 Respiration, ionophore, H+
EDTA −67 −88 Chelator, hydrophilic
Glycine hydroxamate −66 −136 tRNA synthetase
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