Anticholinesterse and antioxidant investigations of crude extracts, subsequent fractions, saponins and flavonoids of atriplex laciniata L.: potential effectiveness in Alzheimer’s and other neurological disorders

Table 3 AChE & BChE inhibitiory potentials of plant extracts using galanthamine as standard

Samples	Concentrations ( μ g/mL)	Percent Inhibition (AChE)	AChE IC ₅₀ ( μ g/mL)	Percent inhibition (BChE)	BChE IC ₅₀ ( μ g/mL)
Al.MeF	1000	78.65 ± 0.70^***	280	82.68 ± 0.60^***	220
	500	67.25 ± 0.55^***		69.42 ± 0.60^***
	250	47.21 ± 0.62^***		52.51 ± 0.66^***
	125	31.55 ± 0.56^***		41.27 ± 0.62^***
Al.HxF	1000	72.51 ± 0.62^***		64.36 ± 0.61^***	400
	500	63.44 ± 0.44^***	310	53.40 ± 0.52^***
	250	42.46 ± 0.47^***		39.46 ± 0.60^***
	125	23.68 ± 0.64^***		22.52 ± 0.49^***
Al.CfF	1000	67.42 ± 0.46 ^***		83.49 ± 0.46^***	160
	500	53.53 ± 0.41^***	390	72.48 ± 0.50^***
	250	40.62 ± 0.69^***		59.59 ± 0.66^***
	125	18.46 ± 0.67^***		45.57 ± 0.63^***
Al.EaF	1000	77.45 ± 0.55 ^***		74.41 ± 0.60^***	260
	500	57.63 ± 0.64^***	270	61.53 ± 0.71^***
	250	48.45 ± 0.66 ^***		49.45 ± 0.77^***
	125	36.36 ± 0.50^***		31.75 ± 0.58^***
Al.WtF	1000	72.44 ± 0.58^***		78.62 ± 0.36^***	210
	500	60.51 ± 0.59^***	263	69.22 ± 0.47^***
	250	48.62 ± 0.70^***		52.52 ± 0.71^***
	125	31.47 ± 0.55^***		43.52 ± 0.70^***
Al.SPF	1000	84.36 ± 0.64 ^***		80.37 ± 0.54^***	120
	500	77.34 ± 0.65^***	90	73.44 ± 0.50^***
	250	65.58 ± 0.67^***		62.51 ± 0.72^***
	125	53.60 ± 0.50^***		49.28 ± 0.61^***
Al.FLVF	1000	88.31 ± 0.57^ns		85.46 ± 0.62^***	100
	500	79.46 ± 0.63 ^*	70	76.58 ± 0.69^***
	250	67.53 ± 0.49 ^***		65.68 ± 0.42^***
	125	59.38 ± 0.50^ns		52.46 ± 0.72^***
Galanthamine	1000	91.37 ± 0.65		94.50 ± 0.71	47
	500	83.33 ± 0.55	52	85.47 ± 0.59
	250	73.31 ± 0.60		71.72 ± 0.51
	125	61.32 ± 0.54		65.37 ± 0.69

Result expressed as % inhibition (mean ± SEM of n = 3) and IC50 values. Values significantly different as compared to positive control, *:P < 0.05, ***:P < 0.001, ns: P >0.05.

ISSN: 0717-6287