In vitro antioxidant capacity and free radical scavenging evaluation of active metabolite constituents of Newbouldia laevis ethanolic leaf extract

Table 4 IC ₅₀ values of Newbouldia laevis scavenging activity and reference compounds

Activity	*N. Laevis* IC ₅₀	Reference	IC ₅₀
DPPH	51.4^#	Ascorbic acid	55.4 ± 20.12**
Hydroxyl radical (OH^.)	497.21 ± 3.65^#	Mannitol	571.45 ± 20.12**
Nitric oxide radical (NO)	92.42 ± 2.73^#	Curcumin	90.82 ± 4.75 (6)**
Superoxide anion (O₂ ^.-)	57.08 ± 1.22^#	Quercetin	42.06 ± 1.35**
Peroxynitrite (ONOO⁻)	1210.83 ± 23.85^#	Gallic acid	876.24 ± 56.96 (6)**
Singlet oxygen (¹O₂)	510.65 ± 9.54	Lipoic acid	46.15 ± 1.16 (6) *
Hypochlorous acid (HOCl)	276.04 ± 12.01^#	Ascorbic acid	235.95 ± 5.75 (6)**
Iron Chelating	1225.05 ± 298.1	EDTA	1.27 ± 0.05 (6)**

Units of IC₅₀ for all activities are μg/ml. Data are expressed as mean ± S.D.
EDTA = Ethylenediamine tetraacetic acid. ^#indicates no significant difference where *p < 0.01 and **p < 0.001.

ISSN: 0717-6287