The effect of EMS on phosphorylation of MAPK in TNF-α-induced HDFs. The phosphorylation of ERK, p38, and JNK was analyzed by western blot analysis. As controls, cells were treated with 10 μM PD98059 (an ERK inhibitor) (A), SB203580 (a p38 inhibitor) (B), or SP600125 (a JNK inhibitor) (C). (D) The relative expression ratio of each p-ERK, p-p38 and p-JNK was calculated after densitometric analysis using image J software and normalization to each ERK, p38 and JNK signal. *p < 0.05 compared with non-treated control group.