Figure 2From: Effects of Er-Miao-San extracts on TNF-alpha-induced MMP-1 expression in human dermal fibroblasts The effect of EMS on TNF-α-induced MMP-1 mRNA expression in HDFs. HDFs were pre-treated with 250–500 μg/ml EMS (A), 250 μg/ml CP and 250 μg/ml RA for 3 h (B), and subsequently stimulated with 10 ng/ml TNF-α for 4 h. The level of MMP-1 mRNA was determined by qRT-PCR. Independent experiments were performed in triplicate (*p < 0.05).Back to article page