Table 1 Primer used for real-time PCR
From: The TLR7 agonist Imiquimod promote the immunogenicity of msenchymal stem cells
Gene | Forward primer | Reverse primer | GenBank number |
|---|---|---|---|
IL-1β | ACGAATCTCCGACCACCACT | CCATGGCCACAACAACTGAC | M15330 |
IL-6 | GACCCAACCACAAATGCCA | GTCATGTCCTGCAGCCACTG | M14584 |
IL-8 | CTGGCCGTGGCTCTCTTG | CCTTGGCAAAACTGCACCTT | NM_000584 |
IL-12 | CGGTCATCTGCCGCAAA | CAAGATGAGCTATAGTAGCGGTCC | M65272 |
IFN-β | CAGCAATTTTCAGTGTCAGAAGCT | TCATCCTGTCCTTGAGGCAGT | M28622 |
NF-κB | AGAGTGCTGGAGTTCAGGATA | AAGGTGGATGATTGCTAAGTGT | AJ271718 |
TGF-β | TATCGACATGGAGCTGGTGAAG | CAGCTTGGACAGGATCTGGC | X02812 |
TNF-α | GGTGCTTGTTCCTCAGCCTC | CAGGCAGAAGAGCGTGGTG | M10988 |
CXCL6 | GCTGAGAGTAAACCCCAAAACG | GGAGCACTGCGGGCC | NM_002993 |
CCL7 | CTGCTCTCCAGCGCTCTCA | GTAAGAAAAGCAGCAGGCGG | NM_002984 |
CCL15 | AGCAGAGGCTGGAGAGCTACA | GGGTCAGCACAGATCTCCTTGT | NM_006273 |
CCL26 | CCTCTCCTGCCTCATGCTTATT | CTCTGTCTCTGCATCATTTGTGAA | U58914 |
Lin28 | TGCTGTCGAGGGATGGATA | CCACCCAATGCGTTCTATTG | NM_024674 |
Otx2 | TCGGGCGCAGCTAGATGT | TGTCTGGGTACCGGGTCTTG | NM_001270525 |
Sox2 | CCCCTTTATTTTCCGTAGTTGTAT | GATTCTCGGCAGACTGATTCAA | NM_003106 |
Sox17 | TGGCGCAGCAGAATCCA | CGACTTGCCCAGCATCTTG | NM_022454 |
GAPDH | GAAGGTGAAGGTCGGAGTC | GAAGATGGTGATGGGATTTC | J04038 |