Investigations of anticholinestrase and antioxidant potentials of methanolic extract, subsequent fractions, crude saponins and flavonoids isolated from Isodon rugosus

Table 2 Percent AChE and BChE inhibition potentials of I. rugosus at various tested concentrations

Samples	Concentrations (μg/ml)	Percent AChE (mean ± SEM)	AChE	Percent	BChE
			IC₅₀	BChE	IC₅₀
			(μg/ml)	(mean ± SEM)	(μg/ml)
Ir.Sp	1000	77.85 ± 0.56***	75	69.48 ± 0.74***	210
	500	71.64 ± 0.75***		62.62 ± 0.40***
	250	62.58 ± 0.77***		51.60 ± 0.46***
Ir.Cr	1000	71.75 ± 0.63***	140	68.68 ± 0.49***	145
	500	63.58 ± 0.70***		61.72 ± 0.66***
	250	56.61 ± 0.53***		55.46 ± 0.63***
Ir.Hex	1000	64.79 ± 0.62***	630	62.61 ± 0.77***	380
	500	45.45 ± 0.49***		54.60 ± 0.80***
	250	43.75 ± 0.58***		43.83 ± 0.56***
Ir.Cf	1000	83.73 ± 0.64***	50	88.55 ± 0.77^ns	70
	500	79.47 ± 0.56***		83.65 ± 0.77^ns
	250	67.81 ± 0.60***		67.58 ± 0.74***
Ir.EtAc	1000	71.74 ± 0.61***	170	76.37 ± 0.68***	52
	500	69.68 ± 0.60***		72.66 ± 0.78***
	250	52.63 ± 0.76***		63.62 ± 0.74***
Ir.Aq	1000	66.79 ± 0.63***	340	71.62 ± 0.74***	305
	500	59.67 ± 0.61***		63.86 ± 0.60***
	250	41.69 ± 0.77***		44.48 ± 0.64***
Ir.Flv	1000	87.44 ± 0.51***	45	82.53 ± 0.71***	40
	500	73.76 ± 0.58***		76.52 ± 0.68***
	250	71.54 ± 0.50***		68.51 ± 0.77***
Galanthamine	1000	96.00 ± 0.30	20	88.61 ± 0.43	47
	500	91.26 ± 1.27		83.25 ± 1.40
	250	82.91 ± 1.30		74.03 ± 0.86

The data is represented as mean ± SEM, (N = 3). Values significantly different as compare to positive control, *P < 0.05, **P < 0.01, ***P < 0.001.
Abbreviations: Ir.Cr Crude methanolic extract, Ir.Hex n-hexane fraction, Ir.Cf Chloroform fraction, Ir.EtAc Ethyl acetate fraction, Ir.Aq Aqueous fraction, Ir.Flv Crude flavonoids, Ir.Sp Crude Saponins.

ISSN: 0717-6287