Figure 1

The comparison between the simultaneous and direct assays. a) The WST-1 measurement obtained 2 h after the addition of BrdU showed that there was no significant difference among the different dosages of the MRP-14 protein (P > 0.05). b) The BrdU measurement performed after WST-1 assay showed that there was a significant difference among the different groups (P < 0.05). The protein dosages of 100, 200, 400 and 800 ng/ml significantly inhibited the proliferation of ASM cells at 24 h (P < 0.05). c) The WST-1 measurement was conducted directly 24 h after the addition of the MRP-14 protein at concentrations of 1, 10, 50, 100, 200, 400 and 800 ng/ml. The result manifested that there was no significant difference among the different dosages of the MRP-14 protein (P > 0.05). d) The direct BrdU measurement was obtained 24 h after the addition of the MRP-14 protein at concentrations of 1, 10, 50, 100, 200, 400 and 800 ng/ml. The result showed that the protein concentrations of 200, 400 and 800 ng/ml significantly inhibited the proliferation of ASM cells at 24 h (P < 0.05). *indicates a significant difference compared with the result obtained with 1 ng/ml (P < 0.05). All of the data were expressed as mean ± SD, n = 4.