Table 1 Sequences and accession numbers for forward and reverse primers used in Real-Time PCR
From: Expression of non-neuronal cholinergic system in maxilla of rat in vivo
Name | Genbank No. | Forward | Reverse |
|---|---|---|---|
CarAT | NM_001004085 | ATTGTCGCTCTTGTGGACC | TCTGTTTGGCCTTCTCTATGTC |
OCTN2 | NM_019269 | CGATCCCAGTGAGTTACAAGAC | GAGAAAGTCCGAAGTAGCCC |
OCT1 | NM_012697 | TGGCCGTAAGCTCTGTCTCT | TCAAGGTATAGCCGGACACC |
OCT3 | NM_019230 | CAATGGGAAACACCTCTCGT | ATACACCACGGCACTTGTGA |
SLC22a4 | NM_022270 | CTGGGAGTACAGCAAGGA | GAAGGAGCCACAGAGAAC |
SLC25a20 | NM_053965 | AACCCATCAGTCCGCTTAAG | TGGTCCCAGAGTACATAGGTG |
VAChT | X80395 | GCCACATCGTTCACTCTCTTG | CGGTTCATCAAGCAACACATC |
AChE | S50879 | CTTCTCCCACACCTGTCC | CTGCTTCCTGGTAGAGCC |
BChE | NM_022942 | ACCTAACCTTGAACACAGAGAAG | TTCCACTCTTGCTCCCTTTC |
α1 | NM_024485 | GTCACCCACTTTCCCTTCGA | CAGGTCGGGCTGGTCACTT |
α2 | NM_133420 | CGCTGGTCATCCCACTCAT | GGGAGCGGTGGTGTACATTG |
α3 | NM_052805 | TCCAGTTTGAGGTGTCCATG | CTTGGTAGTCAGAGGGTTTCC |
α4 | NM_024354.1 | CGCATCCCCTCTGAACTCAT | CACTGCACCCTTCCGTCATA |
α5 | NM_017078 | TGGACGCAACCAGCAAACTA | TATGTCCACGAGCCGAATTTC |
α7 | AY574256 | GCTGGTTCCCTTTTGATG | CTCCGTTGGGGATATAGC |
α10 | AF196344 | CAGTCTCTCCCCAAAGTG | GAGGTGGGCTTTAGATCC |
β1 | X74833 | TGGTTGTGGACCGTCTTTTTC | ATGACCGGAGGGTCCTCAAG |
β2 | L31622 | TTCCTGCTGCTCATCTCCAA | CGCTGGTGACGATGGAGAA |
β3 | NM_133597.1 | TGGAAACACTCTGCGCTTGA | TCCTGCAGTGGCCGTAAGA |
β4 | AY574260 | TGCTGGCACTCACGTTCTTC | AAGGTGACCAGCACCATGGT |
γ | NM_019145 | CACCTACTTCCCCTTCGATTG | ATTCTCTGTGAAAGCCTCGG |
M1 | NM_080773 | TGGTTTCCTTCGTTCTCTGG | GAGGAACTGGATGTAGCACTG |
M2 | J03025 | CCACTCCAGAGATGACAACT | GGCTACAACGTTCTGCTTT |
M3 | M16407 | GGACTGTGGATGTGGAGAG | CGAGGAGTTGGTGTCAGA |
M4 | NM_031547 | CCCGCCGCACTACTAAGATG | CCTCTTGCCCACCACAAACT |
M5 | M22926 | CAGCTGCTGCTCACAGACTCA | GGGAAGGAACAGGGCATGAT |
β-actin | NM_031144 | CTTCAACACCCCAGCCATGT | CAGAGGCATACAGGGACAACA |
18S | M11188 | GCGGTTCTATTTTGTTGG | AATGCTTTCGCTCTGGTC |