Effects of different treatments on the growth and the colony formation in Hep3B cells. (A) The proliferation of Hep3B cells was measured using WST-1 kit. The growth curve of Hep3B cells was shown for each group. The proliferation was assayed in triplicates at 0, 24, 48 and 72 hour post-transfection of siRNAs. (B) Effects of different treatments on the inhibition of cell proliferation were confirmed by the total numbers of colony. (C) Representative images of the colony formation assay were shown. Values were given as mean value ± standard deviation (SD) of triplicate. **p < 0.01, *p < 0.05 compared to untreated cell group; #p < 0.05 compared to siRNA cocktail treated cell group.