Effects of different treatments on ANG2 expression in HUVECs cultured with Hep3B supernatant. HUVEC complete medium (without VEGF) was used as a negative control. (A) ANG2 mRNA expression levels were showed by Real-time qRT-PCR analysis. (B) ANG2 protein in HUVECs was determined by Western Blot analysis. (C) Densitometric analysis of ANG2 protein was made relative to β-actin. Values were given as mean value ± standard deviation (SD) of triplicate. *p < 0.05 compared to untreated cell group.