Effects of pre-designed siRNAs treatments on VEGF and KSP mRNA expression in Hep3B cells. Cells were transfected with siRNAs. Total RNA was extracted from cells at 72 hours after siRNA transfection. The mRNA relative level of VEGF (A) and KSP (B) with siRNAs treatments in Hep3B cells by Real-time qRT-PCR. The mRNA expressions of VEGF and KSP were normalized with β-actin. Values were given as mean value ± standard deviation (SD) of triplicate. **p < 0.01 and *p < 0.05 compared to untreated cell group.