Extraction of saponins and toxicological profile of Teucrium stocksianum boiss extracts collected from District Swat, Pakistan

Table 2 Concentration dependent phytotoxic potential of crude methanolic extracts of T. stocksianum and its sub-fractions against Lamna minor

Sample	Conc; 1000 μg/ml	Conc; 100 μg/ml	Conc; 10 μg/ml	LC₅₀μg/ml
Crude saponins	32.99 ± 2.502%	19.99 ± 1.925%	7.77 ± 1.113%	1350
Crude methanolic extract	95.56 ± 1.10%	71.10 ± 1.10%	27.76 ± 1.90%	30
n -hexane fraction	96.67 ± 1.90%	82.23 ± 2.90%	51.10 ± 1.10%	9.8
Chloroform fraction	93.33 ± 1.90%	56.66 ± 1.90%	42.23 ± 2.90%	50
Ethyl acetate fraction	93.33 ± 1.10%	63.33 ± 1.10%	33.33 ± 1.90%	48
Aqueous fraction	55.55 ± 2.223%	37.77 ± 1.113%	18.88 ± 1.113%	710

Conc; concentration. Values are expressed as the mean ± SEM of three independent observations. Standard drug; Paraquat, LD₅₀ = 0.90 μg/ml.

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