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Table 1 PCR characteristic of primers for constitutive and growth factors genes evaluated in the present study

From: Growth differentiation factor 9 and bone morphogenetic protein 15 expression in previtellogenic oocytes and during early embryonic development of Yellow-tail Kingfish Seriola lalandi

Gene Primer sequences (5′-3′) Tm (°C) Efficency Amplicon (bp)
β-actin F: AGGGAAATCGTGCGTGACAT 57 2,04 191
  R: GCTGAAGTTGTTGGGCGTTT    
tubulin F: TCATCAAGATTATCAGGAGGCG 55 1,98 113
  R: GGAAGCATACACCATGTAGAGG    
gdf9 F: ACGAATGCATGTGCCTTGTA 55 2,01 186
  R: GCTTCTCGTAAATGATGTTCTG    
bmp15 F: GGCAAAGAGAGCCTGGTCACC 59 1,99 218
  R: GAAGGTGGAGTACAGGGAGCT