Table 1 PCR characteristic of primers for constitutive and growth factors genes evaluated in the present study
Gene | Primer sequences (5′-3′) | Tm (°C) | Efficency | Amplicon (bp) |
|---|---|---|---|---|
β-actin | F: AGGGAAATCGTGCGTGACAT | 57 | 2,04 | 191 |
R: GCTGAAGTTGTTGGGCGTTT | ||||
tubulin | F: TCATCAAGATTATCAGGAGGCG | 55 | 1,98 | 113 |
R: GGAAGCATACACCATGTAGAGG | ||||
gdf9 | F: ACGAATGCATGTGCCTTGTA | 55 | 2,01 | 186 |
R: GCTTCTCGTAAATGATGTTCTG | ||||
bmp15 | F: GGCAAAGAGAGCCTGGTCACC | 59 | 1,99 | 218 |
R: GAAGGTGGAGTACAGGGAGCT |