Phytochemical and toxicological investigations of crude methanolic extracts, subsequent fractions and crude saponins of Isodon rugosus

Table 3 Concentration dependent cytotoxic effect of the Ir.Cr extract of I. rugosus and its fractions against Artemia salina

Extracts	Total treated	Dose (μg/ml)	% Lethality (mean ± SEM)	LC₅₀(μg/ml)
Ir.Cr	30	1000	71.10 ± 1.1^***	220
		100	46.67 ± 3.3^***
		10	37.77 ± 1.1^***
Ir.Hex	30	1000	62.23 ± 2.9^***	120
		100	48.90 ± 1.1^***
		10	40.00 ± 1.9^***
Ir.Chf	30	1000	76.67 ± 3.3^***	12
		100	62.23 ± 2.9 ^ns
		10	48.90 ± 1.1 ^ns
Ir.EtAc	30	1000	74.43 ± 2.2^***	315
		100	57.77 ± 2.2^*
		10	43.33 ± 1.9^*
Ir.Aq	30	1000	65.57 ± 1.1^***	60
		100	53.33 ± 1.9^***
		10	42.23 ± 1.1^**
Ir.Sp	30	1000	92.23 ± 1.1	10
		100	66.67 ± 1.9
		10	52.23 ± 1.1

Standard drug; Etoposide, LC₅₀ = 9.8 μg/ml. Values represents % lethality and are expressed as mean ± SEM; *P <0.05, **P <0.01, ***P <0.001.

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