Phytochemical and toxicological investigations of crude methanolic extracts, subsequent fractions and crude saponins of Isodon rugosus

Table 2 Phytotoxic effect of the crude extract of I. rugosus and its fractions against radish seeds

Samples	Conc. (μg/ml)	Root length inhibition% (mean ± SEM)	IC₅₀(μg/ml)	Seeds inhibition% (mean ± SEM)	IC₅₀(μg/ml)
Ir.Cr	1000	72.00 ± 0.20^***	25	58.68 ± 3.52^***	52
	100	63.15 ± 0.91^***		54.49 ± 0.47^***
	10	45.15 ± 0.61^***		39.51 ± 0.58^***
Ir.Hex	1000	80.40 ± 0.68^***	29	56.00 ± 4.60^***	410
	100	77.33 ± 0.64^***		47.26 ± 0.61^***
	10	47.26 ± 0.64^***		41.55 ± 0.61^***
Ir.Chf	1000	88.40 ± 1.36^*	0.1	89.32 ± 3.52 ^ns	0.1
	100	71.37 ± 0.74^***		73.59 ± 0.58 ^ns
	10	66.51 ± 0.39^*		68.37 ± 0.62 ^ns
Ir.EtAc	1000	80.40 ± 0.68^***	0.1	81.32 ± 5.80 ^ns	0.1
	100	74.55 ± 0.51^***		71.55 ± 0.65^*
	100	74.55 ± 0.51^***		59.44 ± 0.58^**
	10	61.55 ± 0.67^***		59.44 ± 0.58^**
Ir.Aq	1000	40.40 ± 0.44^***	1830	49.32 ± 3.52^***	1045
	100	33.67 ± 0.55^***		41.55 ± 0.64^***
	10	30.55 ± 0.64^***		33.62 ± 0.61^***
Ir.Sp	1000	91.33 ± 0.62	0.1	93.55 ± 0.64	0.1
	100	84.52 ± 0.48		81.44 ± 0.67
	10	69.51 ± 0.79		66.59 ± 0.52
Negative control		–		–

Results expressed as average seeds inhibition and Inhibition of root length (mean ± SEM n = 3) and IC₅₀. Values significantly different as compared to Standard drug; *P <0.05, **P <0.01, ***P <0.001.

ISSN: 0717-6287