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Figure 2 | Biological Research

Figure 2

From: Loop-mediated isothermal amplification assays for screening of bacterial integrons

Figure 2

Determination of LAMP amplicons by gel electrophoresis, as well as observation directly by naked eye and under UV light in combination with Sybr Green stain. A: Monitoring of LAMP amplification by gel electrophoresis under different time points: lane 1–7, 8–14, 15–21 referring to LAMP assays of intI1, intI2 and intI3, respectively. Lane 1, 8, 15: DNA Marker; lane 2, 9, 16: 15 min; lane 3, 10, 17: 30 min; lane 4, 11, 18: 45 min; lane 5, 12, 19: 60 min; lane 6, 13, 20: 75 min; lane 7, 14, 21: 90 min. B: LAMP products were visually detected by the turbidity derived from the white precipitate of magnesium pyrophosphate. C: LAMP products dyed with Sybr Green I were visually detected by examining color changes with the naked eye. Assays were performed at 65°C for 45 min.

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