Figure 2From: Loop-mediated isothermal amplification assays for screening of bacterial integronsDetermination of LAMP amplicons by gel electrophoresis, as well as observation directly by naked eye and under UV light in combination with Sybr Green stain. A: Monitoring of LAMP amplification by gel electrophoresis under different time points: lane 1–7, 8–14, 15–21 referring to LAMP assays of intI1, intI2 and intI3, respectively. Lane 1, 8, 15: DNA Marker; lane 2, 9, 16: 15 min; lane 3, 10, 17: 30 min; lane 4, 11, 18: 45 min; lane 5, 12, 19: 60 min; lane 6, 13, 20: 75 min; lane 7, 14, 21: 90 min. B: LAMP products were visually detected by the turbidity derived from the white precipitate of magnesium pyrophosphate. C: LAMP products dyed with Sybr Green I were visually detected by examining color changes with the naked eye. Assays were performed at 65°C for 45 min.Back to article page