Figure 3

Apoptosis evaluation by flow-cytometry of DOK cell line subjected to PDT. A) Apoptosis evaluation for triplicates repeated in four identical experiments. Annexin V-FITC and PI (red) quantified early, late apoptosis and necrosis: An-Pi- viable cells; An + Pi- early apoptotic cells; An + Pi + late apoptotic/necrotic cells. Cell samples are from the same batches that were subjected post-irradiation to Protein microarray analysis. B) Example of flow cytometry registration of un-loaded (control) and loaded cells with AlS₂Pc and subjected to PDT.