Figure 6

The amount of ADAM17 at the cell surface and the proportion of cells with ADAM17 at the cell surface are stage-specific. Seminiferous tubules from adult rat testes were isolated and dissected into the different segments (Pale, Weak, Strong and Dark). After disaggregation, the live cells were labelled with the antibodies against ADAM17 and then with the secondary antibody FITC. The intensity and the percentage of positive cells were determined by flow cytometry. The figure shows (A) the histogram, (B) the percentage of fluorescence intensity (amount of ADAM17 at the cell surface), (C) the dot plot and (D) the percentage of positive cells according to the different segments. The values represent the mean ± S.E.M. of three different experiments (**p < 0.01, ***p < 0.05).